- iPSC Core Research

The mission of induced Pluripotent Stem Cells (iPSC) Core is to accelerate stem cell research by providing investigators consultation, technical services and training in human pluripotent stem cell technology. The major services that iPSC Core currently provides include (1) generation of human iPSCs from fibroblast cells, CD34+ hematopoietic stem/progenitor cells, and peripheral blood mononuclear cells (PBMCs) using non-integration methods; (2) CRISPR/Cas9 mediated human iPSC gene knockout, gene correction, and AAVS1 safe harbor transgene knockin; (3) human iPSC-cardiomyocyte (CM) differentiation. The Core also provides control iPSC lines and validated iPSC culture reagents. In addition to providing services and consultations to NIH researchers, the Core staff collaborate with investigators to work on custom and exploratory projects. Research conducted by the Core seeks to better understand the mechanisms underlying pluripotent stem cell self-renewal and differentiation to optimize culture conditions and operating procedures.

Selected Publications

Prolyl hydroxylation regulates protein degradation, synthesis, and splicing in human induced pluripotent stem cell-derived cardiomyocytes.

Authors: Stoehr A, Yang Y, Patel S, Evangelista AM, Aponte A, Wang G, Liu P, Boylston J, Kloner PH, Lin Y, Gucek M, Zhu J, Murphy E
Cardiovasc Res 2016 Jun 01;110(3):346-58
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Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes.

Authors: Francis KR, Ton AN, Xin Y, O'Halloran PE, Wassif CA, Malik N, Williams IM, Cluzeau CV, Trivedi NS, Pavan WJ, Cho W, Westphal H, Porter FD
Nat Med 2016 Apr 01;22(4):388-96
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Treatment Paradigms for Retinal and Macular Diseases Using 3-D Retina Cultures Derived From Human Reporter Pluripotent Stem Cell Lines.

Authors: Kaewkhaw R, Swaroop M, Homma K, Nakamura J, Brooks M, Kaya KD, Chaitankar V, Michael S, Tawa G, Zou J, Rao M, Zheng W, Cogliati T, Swaroop A
Invest Ophthalmol Vis Sci 2016 Apr 01;57(5):ORSFl1-ORSFl11
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Transcriptome Dynamics of Developing Photoreceptors in Three-Dimensional Retina Cultures Recapitulates Temporal Sequence of Human Cone and Rod Differentiation Revealing Cell Surface Markers and Gene Networks.

Authors: Kaewkhaw R, Kaya KD, Brooks M, Homma K, Zou J, Chaitankar V, Rao M, Swaroop A
Stem Cells 2015 Dec 01;33(12):3504-18
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Eradication of B-ALL using chimeric antigen receptor-expressing T cells targeting the TSLPR oncoprotein.

Authors: Qin H, Cho M, Haso W, Zhang L, Tasian SK, Oo HZ, Negri GL, Lin Y, Zou J, Mallon BS, Maude S, Teachey DT, Barrett DM, Orentas RJ, Daugaard M, Sorensen PH, Grupp SA, Fry TJ
Blood 2015 Jul 30;126(5):629-39
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