Title: Blood Diagnostics: Blood will tell!
Recent advances in omic tools (genomics, proteomics, transcriptomics and metabolomics and their critical metabolites)-have created a set of tools box that can use small blood volumes for ultra-sensitive detection of key biomarkers that will allow creation of pathophysiological molecular finger prints. This will be an easy, fast, inexpensive and sensitive tool for disease diagnostic and prediction on a Nano scale. One can use blood diseases as pilots to explore such technologies. How early can the technology enable detect and predict (for example), early onset stroke, pulmonary hypertension or a vasculopathy in SCD-Sickle cell disease or in certain BMFS-Bone marrow failure disorders, be able to predict early onset of cancers (common in these disorders) and stroke in a thrombotic event. Although the proof of concept for the technology has been shown to be available, there is an unmet need for early diagnosis. This workshop will help explore further the technologies required for creation of key markers and molecular fingerprints for an early detection.
Objective of Workshop: White paper outlining challenges and opportunities and how NHLBI can use this to provide opportunities for research developments.
Session I: Lead: Garry Nolan-
Limitations and opportunities for CELL based diagnostic approaches
Session II Lead: John Stamatoyannopoulos-
Challenges at the interface of high throughput technologies
Session 3: Lead: Shankar Subramaniam-
Markers and mechanisms from blood constituents – implications for pathology.
Session I- State of the field- Innovations: What is out there; what do we need;
How can we determine which biomarker or a combination of, will have the greatest diagnostic power?
Session II- Platforms, Methods and Technologies- Identifying markers from blood/serum: (metabolomics, microRNA, cDNA & cRNA), and (mass spec (MRM (multiple reaction monitoring mass spectrometry), electrophoretic methods, Nano string) etc. How to determine an integrated quantification method to increase the precision, robustness, and resolution of protein measurement in human plasma samples?
Session III- Challenges and opportunities- what are the bottle necks and research and method innovation in blood-related biomedicine? Addressing Unmet Diagnostic Needs- Redefining diagnostics requires innovation on several fronts: informatics & analysis, new technology, and collaboration across disciplines.
SESSION IV- How to Build a New Diagnostics Ecosystem & Execute: Transform blood into a window to distinguish health from disease; Disease diagnostics, assess drug toxicity, assess wellness?
Recommendations to NHLBI
The overall goal is to create large-scale systems medicine diagnostics that can gather a wealth of information – and then make sense of that information so that physicians can better diagnose disease…. A long-term goal!!