Research

Research
- Biophysics Core Research

The Biophysics Core’s mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access. We assist researchers with comprehensive biophysical characterization of biomolecules, including proteins, polynucleotides, nanoparticles, and their cognate ligands.

The Biophysics Core has the resources and expertise to study oligomeric states of biomolecular assemblies and to perform measurements of affinity, stoichiometry, kinetics, and thermodynamics of interactions between proteins, DNA, RNA and small molecules. The Core facility is also equipped with instrumentation for the characterization of nanoparticles and macromolecules. Biophysical characterization capabilities include measurement of molecular weight, shape, and conformation of biological macromolecules.

Instruments

  • Isothermal Titration Calorimetry (ITC: iTC200 and VP-ITC)
  • Differential Scanning Calorimetry (DSC: VP-DSC and Nano-DSC)
  • Surface Plasmon Resonance (SPR: Biacore 3000)
  • Bio-Layer Interferometry (BLI: Octet RED96)
  • MicroScale Thermophoresis (MST: Monolith NT.115)
  • Analytical Ultracentrifugation (AUC: XLA and XLI)
  • Multi Angle Light Scattering (SEC-MALS/QELS: DAWN Helos II)
  • Dynamic Light Scattering (DLS: Dynapro Nanostar)
  • Circular Dichroism (CD: Jasco J715)
  • Fluorescence (Photon Technology)

Selected Publications

Olfactomedin 4 deletion induces colon adenocarcinoma in Apc(Min/+) mice.

Authors: Liu W, Li H, Hong SH, Piszczek GP, Chen W, Rodgers GP
Oncogene 2016 Mar 14

SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions.

Authors: Zhao H, Piszczek G, Schuck P
Methods 2015 Apr 01;76:137-48

Fixation-resistant photoactivatable fluorescent proteins for CLEM.

Authors: Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, Wang Y, Looger LL
Nat Methods 2015 Mar 01;12(3):215-8, 4 p following 218

Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C.

Authors: Gruschus JM, Jiang Z, Yap TL, Hill SA, Grishaev A, Piszczek G, Sidransky E, Lee JC
Biochem Biophys Res Commun 2015 Feb 20;457(4):561-6

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.

Authors: Zhao H, Ghirlando R, Alfonso C, Arisaka F, Attali I, Bain DL, Bakhtina MM, Becker DF, Bedwell GJ, Bekdemir A, Besong TM, Birck C, Brautigam CA, Brennerman W, Byron O, Bzowska A, Chaires JB, Chaton CT, Cölfen H, Connaghan KD, Crowley KA, Curth U, et. al
PLoS One 2015 Jan 01;10(5):e0126420