The NHLBI is hosting 2021 NHLBI Long Non-Coding RNAs Symposium on March 11-12, 2021. The purpose of the virtual conference is to establish an interactive forum at the NIH to discuss and advance the understanding of lncRNA biology and its translational applications, review emerging insights into the molecular mechanisms that lncRNAs regulate diverse biological processes, highlight the recent progress in the research of lncRNAs as key regulators of human pathophysiology and discuss the potential of the large repertoire of human lncRNAs as therapeutic targets for human disease.