The National Heart, Lung, and Blood Institute (NHLBI) convened a group of multidisciplinary experts for a workshop, “Single-Cell Omics ꟷA New Frontier in the Research of Heart, Lung, and Blood Diseases and Sleep Disorders.” Experts in heart, lung, blood, and sleep diseases (HLBS) research and those in the areas of single-cell omics biology, technology, computational biology and national/international single-cell omics consortiums participated to identify research gaps and opportunities in cardiovascular, pulmonary, hematologic and sleep research.
Review the current state of the art knowledge, analytic tools and technologies in the field of single cell biology and discuss the needs for developing and utilizing these resources for better understanding basic biology and pathology of Heart, Lung, and Blood Diseases and Sleep Disorders.
Specific abnormalities in individual cells for most HLBS are either unknown or not well characterized at the molecular level, which is a major obstacle preventing a better understanding of the pathology at the cellular level resulting in tissue damage. The recent advances in technologies for single-cell omics make it feasible to identify the abnormalities of individual cells in HLBS research.
The workshop identified areas of scientific opportunity that would benefit from the use of single-cell omics approaches. The panel discussed these opportunities in four key areas: 1) the cellular origins of HLBS diseases, 2) cell-to-cell interactions during disease progression, 3) cellular phenotypes that define subtypes or endotypes of HLBS diseases and 4) discovery of novel therapeutic targets/approaches that can stop cellular transition to diseased cell types. In addition, the discussion also covered technological advances in the field, such as the best practices for utilizing single-cell technologies, 3D tissue mapping and image mapping. The expert panel made recommendations on the short-term needs and for long-term projections in HLBS research in the area of single cell biology.
Scientific Gaps and Opportunities
The meeting participants will develop a workshop report, highlighting the meeting’s main objectives and scientific opportunities identified. The report will be published in a peer-reviewed journal.
Weiniu Gan, Ph.D
Division of Lung Diseases
National Heart, Lung, and Blood Institute, NIH
WORKING GROUP PARTICIPANTS
Presenters and Moderators, and Discussants
NIH Staff Attendees