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Rosa Puertollano, Ph.D.


Principal Investigator thumbnail image

Rosa Puertollano
Senior Investigator

P: +1 301 451 2361
F: +1 301 402 1519

Full Publications Listing

RRAG GTPases link nutrient availability to gene expression, autophagy and lysosomal biogenesis.
Martina JA, Puertollano R.
Autophagy. 2013 Jun 1;9(6):928-30.
[Text Abstract on PubMed]

What else is in store for autophagy? Exocytosis of autolysosomes as a mechanism of TFEB-mediated cellular clearance in Pompe disease.
Feeney EJ, Spampanato C, Puertollano R, Ballabio A, Parenti G, Raben N.
Autophagy. 2013 May 6;9(7).
[Text Abstract on PubMed]

Rag GTPases mediate amino acid-dependent recruitment of TFEB and MITF to lysosomes.
Martina JA, Puertollano R.
J Cell Biol. 2013 Feb 18;200(4):475-91.
[Text Abstract on PubMed]

Transcription factor EB (TFEB) is a new therapeutic target for Pompe disease.
Spampanato C, Feeney E, Li L, Cardone M, Lim JA, Annunziata F, Zare H, Polishchuk R, Puertollano R, Parenti G, Ballabio A, Raben N.
EMBO Mol Med. 2013 May ;5(5):691-706.
[Text Abstract on PubMed]

MTORC1 functions as a transcriptional regulator of autophagy by preventing nuclear transport of TFEB.
Martina JA, Chen Y, Gucek M, Puertollano R.
Autophagy. 2012 Jun;8(6):903-14.
[Text Abstract on PubMed]

Autophagy in lysosomal storage disorders.
Lieberman AP, Puertollano R, Raben N, Slaugenhaupt S, Walkley SU, Ballabio A.
Autophagy. 2012 May 1;8(5):719-30.
[Text Abstract on PubMed]

Guidelines for the use and interpretation of assays for monitoring autophagy.
Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, Adeli K, Agholme L, Agnello M, Agostinis P, Aguirre-Ghiso JA, Ahn HJ, Ait-Mohamed O, Ait-Si-Ali S, Akematsu T, Akira S, .
Autophagy. 2012 Apr;8(4):445-544.
[Text Abstract on PubMed]

Transcriptional activation of lysosomal exocytosis promotes cellular clearance.
Medina DL, Fraldi A, Bouche V, Annunziata F, Mansueto G, Spampanato C, Puri C, Pignata A, Martina JA, Sardiello M, Palmieri M, Polishchuk R, Puertollano R, Ballabio A.
Dev. Cell. 2011 Sep 13;21(3):421-30.
[Text Abstract on PubMed]

Mucolipin-3 regulates luminal calcium, acidification, and membrane fusion in the endosomal pathway.
Lelouvier B, Puertollano R.
J. Biol. Chem. 2011 Mar 18;286(11):9826-32.
[Text Abstract on PubMed]

LAPTMs regulate lysosomal function and interact with mucolipin 1: new clues for understanding mucolipidosis type IV.
Vergarajauregui S, Martina JA, Puertollano R.
J. Cell. Sci. 2011 Feb 1;124(Pt 3):459-68.
[Text Abstract on PubMed]

Role of TRP channels in the regulation of the endosomal pathway.
Abe K, Puertollano R.
Physiology (Bethesda). 2011 Feb;26(1):14-22.
[Text Abstract on PubMed]

EDITOR'S CHOICE: Disruption of the Murine Ap2ß1 Gene Causes Nonsyndromic Cleft Palate.
Li W, Puertollano R, Bonifacino JS, Overbeek PA, Everett ET.
Cleft Palate Craniofac. J. 2010 Nov;47(6):566-73.
[Text Abstract on PubMed]

Identification of the penta-EF-hand protein ALG-2 as a Ca2+-dependent interactor of mucolipin-1.
Vergarajauregui S, Martina JA, Puertollano R.
J. Biol. Chem. 2009 Dec 25;284(52):36357-66.
[Text Abstract on PubMed]

The calcium channel mucolipin-3 is a novel regulator of trafficking along the endosomal pathway.
Martina JA, Lelouvier B, Puertollano R.
Traffic. 2009 Aug;10(8):1143-56.
[Text Abstract on PubMed]

TRPMLs: in sickness and in health.
Puertollano R, Kiselyov K.
Am. J. Physiol. Renal Physiol. 2009 Jun;296(6):F1245-54.
[Text Abstract on PubMed]

STAM adaptor proteins interact with COPII complexes and function in ER-to-Golgi trafficking.
Rismanchi N, Puertollano R, Blackstone C.
Traffic. 2009 Feb;10(2):201-17.
[Text Abstract on PubMed]

An essential role for the MAL protein in targeting Lck to the plasma membrane of human T lymphocytes.
Antón O, Batista A, Millán J, Andrés-Delgado L, Puertollano R, Correas I, Alonso MA.
J. Exp. Med. 2008 Dec 22;205(13):3201-13.
[Text Abstract on PubMed]

Autophagic dysfunction in mucolipidosis type IV patients.
Vergarajauregui S, Connelly PS, Daniels MP, Puertollano R.
Hum. Mol. Genet. 2008 Sep 1;17(17):2723-37.
[Text Abstract on PubMed]

Mucolipidosis type IV: the importance of functional lysosomes for efficient autophagy.
Vergarajauregui S, Puertollano R.
Autophagy. 2008 Aug;4(6):832-4.
[Text Abstract on PubMed]

Mucolipin 1 channel activity is regulated by protein kinase A-mediated phosphorylation.
Vergarajauregui S, Oberdick R, Kiselyov K, Puertollano R.
Biochem. J. 2008 Mar 1;410(2):417-25.
[Text Abstract on PubMed]

Mucolipin-2 localizes to the Arf6-associated pathway and regulates recycling of GPI-APs.
Karacsonyi C, Miguel AS, Puertollano R.
Traffic. 2007 Oct;8(10):1404-14.
[Text Abstract on PubMed]

Troyer syndrome protein spartin is mono-ubiquitinated and functions in EGF receptor trafficking.
Bakowska JC, Jupille H, Fatheddin P, Puertollano R, Blackstone C.
Mol. Biol. Cell. 2007 May;18(5):1683-92.
[Text Abstract on PubMed]

Activation of p38 mitogen-activated protein kinase promotes epidermal growth factor receptor internalization.
Vergarajauregui S, San Miguel A, Puertollano R.
Traffic. 2006 Jun;7(6):686-98.
[Text Abstract on PubMed]

Two di-leucine motifs regulate trafficking of mucolipin-1 to lysosomes.
Vergarajauregui S, Puertollano R.
Traffic. 2006 Mar;7(3):337-53.
[Text Abstract on PubMed]

Dynamics of MAL2 during glycosylphosphatidylinositol-anchored protein transcytotic transport to the apical surface of hepatoma HepG2 cells.
de Marco MC, Puertollano R, Martínez-Menárguez JA, Alonso MA.
Traffic. 2006 Jan;7(1):61-73.
[Text Abstract on PubMed]

Interactions of TOM1L1 with the multivesicular body sorting machinery.
Puertollano R.
J. Biol. Chem. 2005 Mar 11;280(10):9258-64.
[Text Abstract on PubMed]

Clathrin-mediated transport: assembly required. Workshop on Molecular Mechanisms of Vesicle Selectivity.
Puertollano R.
EMBO Rep. 2004 Oct;5(10):942-6.
[Text Abstract on PubMed]

The trihelical bundle subdomain of the GGA proteins interacts with multiple partners through overlapping but distinct sites.
Mattera R, Puertollano R, Smith WJ, Bonifacino JS.
J. Biol. Chem. 2004 Jul 23;279(30):31409-18.
[Text Abstract on PubMed]

Interactions of GGA3 with the ubiquitin sorting machinery.
Puertollano R, Bonifacino JS.
Nat. Cell Biol. 2004 Mar;6(3):244-51.
[Text Abstract on PubMed]

Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network.
Puertollano R, van der Wel NN, Greene LE, Eisenberg E, Peters PJ, Bonifacino JS.
Mol. Biol. Cell. 2003 Apr;14(4):1545-57.
[Text Abstract on PubMed]

Adaptor and clathrin exchange at the plasma membrane and trans-Golgi network.
Wu X, Zhao X, Puertollano R, Bonifacino JS, Eisenberg E, Greene LE.
Mol. Biol. Cell. 2003 Feb;14(2):516-28.
[Text Abstract on PubMed]

Arf regulates interaction of GGA with mannose-6-phosphate receptor.
Hirsch DS, Stanley KT, Chen LX, Jacques KM, Puertollano R, Randazzo PA.
Traffic. 2003 Jan;4(1):26-35.
[Text Abstract on PubMed]

Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics.
Wasiak S, Legendre-Guillemin V, Puertollano R, Blondeau F, Girard M, de Heuvel E, Boismenu D, Bell AW, Bonifacino JS, McPherson PS.
J. Cell Biol. 2002 Sep 2;158(5):855-62.
[Text Abstract on PubMed]

Phosphoregulation of sorting signal-VHS domain interactions by a direct electrostatic mechanism.
Kato Y, Misra S, Puertollano R, Hurley JH, Bonifacino JS.
Nat. Struct. Biol. 2002 Jul;9(7):532-6.
[Text Abstract on PubMed]

Structural basis for acidic-cluster-dileucine sorting-signal recognition by VHS domains.
Misra S, Puertollano R, Kato Y, Bonifacino JS, Hurley JH.
Nature. 2002 Feb 21;415(6874):933-7.
[Text Abstract on PubMed]

BENE, a novel raft-associated protein of the MAL proteolipid family, interacts with caveolin-1 in human endothelial-like ECV304 cells.
de Marco MC, Kremer L, Albar JP, Martinez-Menarguez JA, Ballesta J, Garcia-Lopez MA, Marazuela M, Puertollano R, Alonso MA.
J. Biol. Chem. 2001 Jun 22;276(25):23009-17.
[Text Abstract on PubMed]

Sorting of mannose 6-phosphate receptors mediated by the GGAs.
Puertollano R, Aguilar RC, Gorshkova I, Crouch RJ, Bonifacino JS.
Science. 2001 Jun 1;292(5522):1712-6.
[Text Abstract on PubMed]

An intact dilysine-like motif in the carboxyl terminus of MAL is required for normal apical transport of the influenza virus hemagglutinin cargo protein in epithelial Madin-Darby canine kidney cells.
Puertollano R, Martínez-Menárguez JA, Batista A, Ballesta J, Alonso MA.
Mol. Biol. Cell. 2001 Jun;12(6):1869-83.
[Text Abstract on PubMed]

The GGAs promote ARF-dependent recruitment of clathrin to the TGN.
Puertollano R, Randazzo PA, Presley JF, Hartnell LM, Bonifacino JS.
Cell. 2001 Apr 6;105(1):93-102.
[Text Abstract on PubMed]

The MAL proteolipid is necessary for the overall apical delivery of membrane proteins in the polarized epithelial Madin-Darby canine kidney and fischer rat thyroid cell lines.
Martín-Belmonte F, Puertollano R, Millán J, Alonso MA.
Mol. Biol. Cell. 2000 Jun;11(6):2033-45.
[Text Abstract on PubMed]

GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex.
Dell'Angelica EC, Puertollano R, Mullins C, Aguilar RC, Vargas JD, Hartnell LM, Bonifacino JS.
J. Cell Biol. 2000 Apr 3;149(1):81-94.
[Text Abstract on PubMed]

Incorporation of MAL, an integral protein element of the machinery for the glycolipid and cholesterol-mediated apical pathway of transport, into artificial membranes requires neither of these lipid species.
Puertollano R, Menéndez M, Alonso MA.
Biochem. Biophys. Res. Commun. 1999 Dec 20;266(2):330-3.
[Text Abstract on PubMed]

MAL, an integral element of the apical sorting machinery, is an itinerant protein that cycles between the trans-Golgi network and the plasma membrane.
Puertollano R, Alonso MA.
Mol. Biol. Cell. 1999 Oct;10(10):3435-47.
[Text Abstract on PubMed]

Substitution of the two carboxyl-terminal serines by alanine causes retention of MAL, a component of the apical sorting machinery, in the endoplasmic reticulum.
Puertollano R, Alonso MA.
Biochem. Biophys. Res. Commun. 1999 Jun 24;260(1):188-92.
[Text Abstract on PubMed]

The MAL proteolipid is necessary for normal apical transport and accurate sorting of the influenza virus hemagglutinin in Madin-Darby canine kidney cells.
Puertollano R, Martín-Belmonte F, Millán J, de Marco MC, Albar JP, Kremer L, Alonso MA.
J. Cell Biol. 1999 Apr 5;145(1):141-51.
[Text Abstract on PubMed]

Targeting of MAL, a putative element of the apical sorting machinery, to glycolipid-enriched membranes requires a pre-golgi sorting event.
Puertollano R, Alonso MA.
Biochem. Biophys. Res. Commun. 1999 Jan 27;254(3):689-92.
[Text Abstract on PubMed]

A short peptide motif at the carboxyl terminus is required for incorporation of the integral membrane MAL protein to glycolipid-enriched membranes.
Puertollano R, Alonso MA.
J. Biol. Chem. 1998 May 22;273(21):12740-5.
[Text Abstract on PubMed]

Recombinant expression of the MAL proteolipid, a component of glycolipid-enriched membrane microdomains, induces the formation of vesicular structures in insect cells.
Puertollano R, Li S, Lisanti MP, Alonso MA.
J. Biol. Chem. 1997 Jul 18;272(29):18311-5.
[Text Abstract on PubMed]

A study of genetic association between manic-depressive illness and a highly polymorphic marker from the GABRbeta-1 gene.
Puertollano R, Visedo G, Zapata C, Fernández-Piqueras J.
Am. J. Med. Genet. 1997 May 31;74(3):342-4.
[Text Abstract on PubMed]

Caveolin and MAL, two protein components of internal detergent-insoluble membranes, are in distinct lipid microenvironments in MDCK cells.
Millán J, Puertollano R, Fan L, Alonso MA.
Biochem. Biophys. Res. Commun. 1997 Apr 28;233(3):707-12.
[Text Abstract on PubMed]

Structural and biochemical similarities reveal a family of proteins related to the MAL proteolipid, a component of detergent-insoluble membrane microdomains.
Pérez P, Puertollano R, Alonso MA.
Biochem. Biophys. Res. Commun. 1997 Mar 27;232(3):618-21.
[Text Abstract on PubMed]

The MAL proteolipid is a component of the detergent-insoluble membrane subdomains of human T-lymphocytes.
Millán J, Puertollano R, Fan L, Rancaño C, Alonso MA.
Biochem. J. 1997 Jan 1;321 ( Pt 1):247-52.
[Text Abstract on PubMed]

Lack of association between manic-depressive illness and a highly polymorphic marker from GABRA3 gene.
Puertollano R, Visedo G, Saiz-Ruiz J, Llinares C, Fernández-Piqueras J.
Am. J. Med. Genet. 1995 Oct 9;60(5):434-5.
[Text Abstract on PubMed]

Familial cosegregation of manic-depressive illness and a form of hereditary cerebellar ataxia.
Fernández Piqueras J, Santos J, Visedo G, Pérez de Castro I, Puertollano R, Montejo J, Ramo Tello C, Valle J.
Am. J. Med. Genet. 1995 Jun 19;60(3):206-9.
[Text Abstract on PubMed]