NIH Research Festival 2013
Dec 25, 2013
Mukoyama's Lab Group photo (Fall, 2012)
Cherry Blossoms Outing Spring 2013
Mukoyama's Lab Group photo (Fall, 2013)
Structural interdependence of blood vessels and nerves to form coordinated branching networks EC, endothelial cells; VSMC, vascular smooth muscle cells. Modified from Fig. 3 in James & Mukouyama. Semin Cell Dev Biol 22: 1019-1027, 2011.
Nerve-artery alignment in developing skin Sensory nerve (green) align with arteries (red) in the developing mouse skin. The image was published in Cell (Mukouyama et al. Cell109: 693-705, 2002).
Chemokine controls nerve-vessel co-patterning Chemokine receptor Cxcr4 expression (red) was restricted to nerve (green)-associated ephrinB2+ arteries (blue). The image was published in Developmental Cell (Li et al. Dev Cell 24: 359-371, 2013).
Cardiac axons follow coronary vessels in embryonic heart Cardiac sympathetic axons (green) follow VSMC-associated large-diameter coronary vessels in the subepicardium of the dorsal ventricular wall of the developing mouse heart. The image was selected as the cover of the issue in Development (Nam et al. Development 140: 1475-1485, 2013).
Intra-neural hemorrhage in the brain: a mouse model of human CCMs The vascular lumen appears to have ruptured resulting in hemorrhage in Ccm2 conditional mutants as evidenced by erythrocytes (green) outside the vessel enclosure (red). The image was published in Human Molecular Genetics (Cunningham et al. Hum Mol Genet 20: 3198-3206, 2011)
Venous expression of EphB1 in embryonic limb skin Whole-mount triple immunofluorescence analysis of forelimb skin of E17.5 embryos was performed with antibodies to EphB1 (green) in combination with the pan-endothelial cell marker PECAM-1 (blue) and the vascular smooth muscle cell (VSMC) marker alpha SMA (red). By E17.5, arterial vessels are densely covered with VSMCs, whereas less or no VSMC coverage occurs in venous vessels. EphB1 expression is restricted to the venous vasculature. No EphB1 expression is detectable in the arterial and lymphatic vasculature. The image was published in Developmental Dynamics (Li et al. Dev Dyn 2013).
Skin lymphangiogenesis Whole-mount confocal microscopy reveals that Prox1+ (blue)/LYVE-1+ (green) lymphatic vessels sprout toward the dorsal midline in E14.5 mouse dorsal skin. PECAM-1+ blood vessels (red) have reached the midline at this stage. The image was published in Development (James et al. Development 2013).