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Jay R. Knutson, Ph.D.


Principal Investigator thumbnail image

Jay R. Knutson
Senior Investigator

P: +1 301 496 2557
F: +1 301 480 6964

Full Publications Listing

Activation of moesin, a protein that links actin cytoskeleton to the plasma membrane, occurs by phosphatidylinositol 4,5-bisphosphate (PIP2) binding sequentially to two sites and releasing an autoinhibitory linker.
Ben-Aissa K, Patino-Lopez G, Belkina NV, Maniti O, Rosales T, Hao JJ, Kruhlak MJ, Knutson JR, Picart C, Shaw S.
J. Biol. Chem. 2012 May 11;287(20):16311-23.
[Text Abstract on PubMed]

In vivo fluorescence lifetime imaging monitors binding of specific probes to cancer biomarkers.
Ardeshirpour Y, Chernomordik V, Zielinski R, Capala J, Griffiths G, Vasalatiy O, Smirnov AV, Knutson JR, Lyakhov I, Achilefu S, Gandjbakhche A, Hassan M.
PLoS ONE. 2012;7(2):e31881.
[Text Abstract on PubMed]

Optimizing multiphoton fluorescence microscopy light collection from living tissue by noncontact total emission detection (epiTED).
Combs CA, Smirnov A, Chess D, McGavern DB, Schroeder JL, Riley J, Kang SS, Lugar-Hammer M, Gandjbakhche A, Knutson JR, Balaban RS.
J Microsc. 2011 Feb;241(2):153-61.
[Text Abstract on PubMed]

Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates.
Stasevich TJ, Mueller F, Michelman-Ribeiro A, Rosales T, Knutson JR, McNally JG.
Biophys. J. 2010 Nov 3;99(9):3093-101.
[Text Abstract on PubMed]

HIV-1 Nef binds a subpopulation of MHC-I throughout its trafficking itinerary and down-regulates MHC-I by perturbing both anterograde and retrograde trafficking.
Yi L, Rosales T, Rose JJ, Chowdhury B, Chaudhury B, Knutson JR, Venkatesan S.
J. Biol. Chem. 2010 Oct 1;285(40):30884-905.
[Text Abstract on PubMed]

FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy.
Fiammenghi L, Ancarani V, Rosales T, Knutson JR, Petrini M, Granato AM, Pancisi E, Ridolfi L, Ridolfi R, Riccobon A, Neyroz P.
J Transl Med. 2010;8:52.
[Text Abstract on PubMed]

Femtosecond fluorescence spectra of tryptophan in human gamma-crystallin mutants: site-dependent ultrafast quenching.
Xu J, Chen J, Toptygin D, Tcherkasskaya O, Callis P, King J, Brand L, Knutson JR.
J. Am. Chem. Soc. 2009 Nov 25;131(46):16751-7.
[Text Abstract on PubMed]

Conformational heterogeneity and quasi-static self-quenching in DNA containing a fluorescent guanine analogue, 3MI or 6MI.
Wojtuszewski Poulin K, Smirnov AV, Hawkins ME, Balis FM, Knutson JR.
Biochemistry. 2009 Sep 22;48(37):8861-8.
[Text Abstract on PubMed]

Quasi-static self-quenching of Trp-X and X-Trp dipeptides in water: ultrafast fluorescence decay.
Xu J, Knutson JR.
J Phys Chem B. 2009 Sep 3;113(35):12084-9.
[Text Abstract on PubMed]

Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy.
Michelman-Ribeiro A, Mazza D, Rosales T, Stasevich TJ, Boukari H, Rishi V, Vinson C, Knutson JR, McNally JG.
Biophys. J. 2009 Jul 8;97(1):337-46.
[Text Abstract on PubMed]

Molecular dynamics simulations of perylene and tetracene librations: comparison with femtosecond upconversion data.
Rosales T, Xu J, Wu X, Hodoscek M, Callis P, Brooks BR, Knutson JR.
J Phys Chem A. 2008 Jun 26;112(25):5593-7.
[Text Abstract on PubMed]

SNP analysis using CataCleave probes.
Harvey JJ, Brant SR, Knutson JR, Han MK.
J. Clin. Lab. Anal. 2008;22(3):192-203.
[Text Abstract on PubMed]

Ultrafast fluorescence spectroscopy via upconversion applications to biophysics.
Xu J, Knutson JR.
Meth. Enzymol. 2008;450:159-83.
[Text Abstract on PubMed]

Optimization of multiphoton excitation microscopy by total emission detection using a parabolic light reflector.
Combs CA, Smirnov AV, Riley JD, Gandjbakhche AH, Knutson JR, Balaban RS.
J Microsc. 2007 Dec;228(Pt 3):330-7.
[Text Abstract on PubMed]

Quantitative detection of the ligand-dependent interaction between the androgen receptor and the co-activator, Tif2, in live cells using two color, two photon fluorescence cross-correlation spectroscopy.
Rosales T, Georget V, Malide D, Smirnov A, Xu J, Combs C, Knutson JR, Nicolas JC, Royer CA.
Eur. Biophys. J. 2007 Feb;36(2):153-61.
[Text Abstract on PubMed]

Examination of the premelting transition of DNA A-tracts using a fluorescent adenosine analogue.
Augustyn KE, Wojtuszewski K, Hawkins ME, Knutson JR, Mukerji I.
Biochemistry. 2006 Apr 18;45(15):5039-47.
[Text Abstract on PubMed]

Ultrafast fluorescence dynamics of tryptophan in the proteins monellin and IIAGlc.
Xu J, Toptygin D, Graver KJ, Albertini RA, Savtchenko RS, Meadow ND, Roseman S, Callis PR, Brand L, Knutson JR.
J. Am. Chem. Soc. 2006 Feb 1;128(4):1214-21.
[Text Abstract on PubMed]

Distribution of mitochondrial NADH fluorescence lifetimes: steady-state kinetics of matrix NADH interactions.
Blinova K, Carroll S, Bose S, Smirnov AV, Harvey JJ, Knutson JR, Balaban RS.
Biochemistry. 2005 Feb 22;44(7):2585-94.
[Text Abstract on PubMed]

Characterization and applications of CataCleave probe in real-time detection assays.
Harvey JJ, Lee SP, Chan EK, Kim JH, Hwang ES, Cha CY, Knutson JR, Han MK.
Anal. Biochem. 2004 Oct 15;333(2):246-55.
[Text Abstract on PubMed]

Flow cytometric measurement of fluorescence (Förster) resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein using single-laser excitation at 458 nm.
He L, Bradrick TD, Karpova TS, Wu X, Fox MH, Fischer R, McNally JG, Knutson JR, Grammer AC, Lipsky PE.
Cytometry A. 2003 May;53(1):39-54.
[Text Abstract on PubMed]

Rapid segmental and subdomain motions of DNA polymerase beta.
Kim SJ, Beard WA, Harvey J, Shock DD, Knutson JR, Wilson SH.
J. Biol. Chem. 2003 Feb 14;278(7):5072-81.
[Text Abstract on PubMed]

Fluorescence studies of pyrene maleimide-labeled translin: excimer fluorescence indicates subunits associate in a tail-to-tail configuration to form octamer.
Han MK, Lin P, Paek D, Harvey JJ, Fuior E, Knutson JR.
Biochemistry. 2002 Mar 12;41(10):3468-76.
[Text Abstract on PubMed]

Nanosecond dynamics of the single tryptophan reveals multi-state equilibrium unfolding of protein GB1.
Tcherkasskaya O, Knutson JR, Bowley SA, Frank MK, Gronenborn AM.
Biochemistry. 2000 Sep 19;39(37):11216-26.
[Text Abstract on PubMed]

Nanosecond dynamics of tryptophans in different conformational states of apomyoglobin proteins.
Tcherkasskaya O, Ptitsyn OB, Knutson JR.
Biochemistry. 2000 Feb 22;39(7):1879-89.
[Text Abstract on PubMed]

Visual detection of transport-P in peptidergic neurones.
al-Damluji S, Porter D, Krsmanovic LZ, Knutson JR, Kopin IJ.
Br. J. Pharmacol. 1997 Mar;120(5):876-82.
[Text Abstract on PubMed]

Zn2+ promotes the self-association of human immunodeficiency virus type-1 integrase in vitro.
Lee SP, Xiao J, Knutson JR, Lewis MS, Han MK.
Biochemistry. 1997 Jan 7;36(1):173-80.
[Text Abstract on PubMed]

Fluorescence properties of pteridine nucleoside analogs as monomers and incorporated into oligonucleotides.
Hawkins ME, Pfleiderer W, Balis FM, Porter D, Knutson JR.
Anal. Biochem. 1997 Jan 1;244(1):86-95.
[Text Abstract on PubMed]

Critical amino acids in the transcriptional activation domain of the herpesvirus protein VP16 are solvent-exposed in highly mobile protein segments. An intrinsic fluorescence study.
Shen F, Triezenberg SJ, Hensley P, Porter D, Knutson JR.
J. Biol. Chem. 1996 Mar 1;271(9):4819-26.
[Text Abstract on PubMed]

Transcriptional activation domain of the herpesvirus protein VP16 becomes conformationally constrained upon interaction with basal transcription factors.
Shen F, Triezenberg SJ, Hensley P, Porter D, Knutson JR.
J. Biol. Chem. 1996 Mar 1;271(9):4827-37.
[Text Abstract on PubMed]

Characterization of endonucleolytic activity of HIV-1 integrase using a fluorogenic substrate.
Lee SP, Censullo ML, Kim HG, Knutson JR, Han MK.
Anal. Biochem. 1995 May 20;227(2):295-301.
[Text Abstract on PubMed]

Characterization of the tryptophan fluorescence and hydrodynamic properties of rat DNA polymerase beta.
Kim SJ, Lewis MS, Knutson JR, Porter DK, Kumar A, Wilson SH.
J. Mol. Biol. 1994 Nov 25;244(2):224-35.
[Text Abstract on PubMed]

Determination of the monomer-dimer equilibrium of interleukin-8 reveals it is a monomer at physiological concentrations.
Burrows SD, Doyle ML, Murphy KP, Franklin SG, White JR, Brooks I, McNulty DE, Scott MO, Knutson JR, Porter D.
Biochemistry. 1994 Nov 1;33(43):12741-5.
[Text Abstract on PubMed]

Actobindin induces the accumulation of actin dimers that neither nucleate polymerization nor self-associate.
Bubb MR, Knutson JR, Porter DK, Korn ED.
J. Biol. Chem. 1994 Oct 14;269(41):25592-7.
[Text Abstract on PubMed]

A fluorometric assay for DNA cleavage reactions characterized with BamHI restriction endonuclease.
Lee SP, Porter D, Chirikjian JG, Knutson JR, Han MK.
Anal. Biochem. 1994 Aug 1;220(2):377-83.
[Text Abstract on PubMed]

Study of the conformation of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein, by fluorescence spectroscopy.
Neyroz P, Desdouits F, Benfenati F, Knutson JR, Greengard P, Girault JA.
J. Biol. Chem. 1993 Nov 15;268(32):24022-31.
[Text Abstract on PubMed]

Interference of diffusive light waves.
Schmitt JM, Knüttel A, Knutson JR.
J Opt Soc Am A. 1992 Oct;9(10):1832-43.
[Text Abstract on PubMed]

Alternatives to consider in fluorescence decay analysis.
Knutson JR.
Meth. Enzymol. 1992;210:357-74.
[Text Abstract on PubMed]

Interaction of influenza hemagglutinin amino-terminal peptide with phospholipid vesicles: a fluorescence study.
Clague MJ, Knutson JR, Blumenthal R, Herrmann A.
Biochemistry. 1991 Jun 4;30(22):5491-7.
[Text Abstract on PubMed]

Fluorescence of tryptophan dipeptides: correlations with the rotamer model.
Chen RF, Knutson JR, Ziffer H, Porter D.
Biochemistry. 1991 May 28;30(21):5184-95.
[Text Abstract on PubMed]

Steady-state fluorescence and time-resolved fluorescence monitor changes in tryptophan environment in arginase from Saccharomyces cerevisiae upon removal of catalytic and structural metal ions.
Green SM, Knutson JR, Hensley P.
Biochemistry. 1990 Oct 2;29(39):9159-68.
[Text Abstract on PubMed]

Hydrophobic surfaces of tubulin probed by time-resolved and steady-state fluorescence of nile red.
Sackett DL, Knutson JR, Wolff J.
J. Biol. Chem. 1990 Sep 5;265(25):14899-906.
[Text Abstract on PubMed]

Sugar transport by the bacterial phosphotransferase system. Fluorescence studies of subunit interactions of enzyme I.
Han MK, Knutson JR, Roseman S, Brand L.
J. Biol. Chem. 1990 Feb 5;265(4):1996-2003.
[Text Abstract on PubMed]

Fluorescence studies of membrane dynamics and heterogeneity.
Davenport L, Knutson JR, Brand L.
Subcell. Biochem. 1989;14:145-88.
[Text Abstract on PubMed]

Studies of lipid fluctuations using polarized fluorescence spectroscopy.
Davenport L, Wang JZ, Knutson JR.
Prog. Clin. Biol. Res. 1989;292:97-106.
[Text Abstract on PubMed]

Mechanism of fluorescence concentration quenching of carboxyfluorescein in liposomes: energy transfer to nonfluorescent dimers.
Chen RF, Knutson JR.
Anal. Biochem. 1988 Jul;172(1):61-77.
[Text Abstract on PubMed]

Nanosecond time-resolved fluorescence measurements during protein denaturation.
Walbridge DG, Knutson JR, Brand L.
Anal. Biochem. 1987 Mar;161(2):467-78.
[Text Abstract on PubMed]

Global analysis of multiple dye fluorescence anisotropy experiments on proteins.
Beechem JM, Knutson JR, Brand L.
Biochem. Soc. Trans. 1986 Oct;14(5):832-5.
[Text Abstract on PubMed]

Anisotropy decay associated fluorescence spectra and analysis of rotational heterogeneity. 1. Theory and applications.
Knutson JR, Davenport L, Brand L.
Biochemistry. 1986 Apr 8;25(7):1805-10.
[Text Abstract on PubMed]

Anisotropy decay associated fluorescence spectra and analysis of rotational heterogeneity. 2. 1,6-Diphenyl-1,3,5-hexatriene in lipid bilayers.
Davenport L, Knutson JR, Brand L.
Biochemistry. 1986 Apr 8;25(7):1811-6.
[Text Abstract on PubMed]

Excited-state proton transfer of equilenin and dihydroequilenin: interaction with bilayer vesicles.
Davenport L, Knutson JR, Brand L.
Biochemistry. 1986 Mar 11;25(5):1186-95.
[Text Abstract on PubMed]

Studies of membrane heterogeneity using fluorescence associative techniques.
Davenport L, Knutson JR, Brand L.
Faraday Discuss Chem Soc. 1986(81):81-94.
[Text Abstract on PubMed]

Decay-associated fluorescence spectra and the heterogeneous emission of alcohol dehydrogenase.
Knutson JR, Walbridge DG, Brand L.
Biochemistry. 1982 Sep 14;21(19):4671-9.
[Text Abstract on PubMed]

Hindered depolarizing rotations of perylene in lipid bilayers. Detection by lifetime-resolved fluorescence anisotropy measurements.
Lakowicz JR, Knutson JR.
Biochemistry. 1980 Mar 4;19(5):905-11.
[Text Abstract on PubMed]