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John A. Hammer, Ph.D.


Principal Investigator thumbnail image

John A. Hammer
Senior Investigator

P: +1 301 496 8960
F: +1 301 402 1519

Full Publications Listing

Functions of class V myosins in neurons.
Hammer JA 3rd, Wagner W.
J Biol Chem. 2013 Oct 4;288(40):28428-34.
[Text Abstract on PubMed]

Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage.
Yi J, Wu X, Chung AH, Chen JK, Kapoor TM, Hammer JA.
J Cell Biol. 2013 Sep 2;202(5):779-92.
[Text Abstract on PubMed]

Controversy and consensus regarding myosin II function at the immunological synapse.
Hammer JA 3rd, Burkhardt JK.
Curr Opin Immunol. 2013 Jun ;25(3):300-6.
[Text Abstract on PubMed]

Melanoregulin is stably targeted to the melanosome membrane by palmitoylation.
Wu XS, Martina JA, Hammer JA 3rd.
Biochem Biophys Res Commun. 2012 Sep 21;426(2):209-14.
[Text Abstract on PubMed]

Melanoregulin regulates a shedding mechanism that drives melanosome transfer from melanocytes to keratinocytes.
Wu XS, Masedunskas A, Weigert R, Copeland NG, Jenkins NA, Hammer JA.
Proc. Natl. Acad. Sci. U.S.A. 2012 Jul 31;109(31):E2101-9.
[Text Abstract on PubMed]

Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes.
Chen Y, Wang Y, Zhang J, Deng Y, Jiang L, Song E, Wu XS, Hammer JA, Xu T, Lippincott-Schwartz J.
J Cell Biol. 2012 Aug 20;198(4):545-60.
[Text Abstract on PubMed]

Actin retrograde flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells.
Yi J, Wu XS, Crites T, Hammer.
Mol. Biol. Cell. 2012 Mar;23(5):834-52.
[Text Abstract on PubMed]

Walking to work: roles for class V myosins as cargo transporters.
Hammer, Sellers JR.
Nat. Rev. Mol. Cell Biol. 2012 Jan;13(1):13-26.
[Text Abstract on PubMed]

An efficient method for the long-term and specific expression of exogenous cDNAs in cultured Purkinje neurons.
Wagner W, McCroskery S, Hammer JA 3rd.
J Neurosci Methods. 2011 Sep 15;200(2):95-105.
[Text Abstract on PubMed]

Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons.
Wagner W, Brenowitz SD, Hammer.
Nat. Cell Biol. 2011 Jan;13(1):40-8.
[Text Abstract on PubMed]

Aspergillus myosin-V supports polarized growth in the absence of microtubule-based transport.
Zhang J, Tan K, Wu X, Chen G, Sun J, Reck-Peterson SL, Hammer JA 3rd, Xiang X.
PLoS One. 2011 01 ;6(12):e28575.
[Text Abstract on PubMed]

Imaging of lytic granule exocytosis in CD8+ cytotoxic T lymphocytes reveals a modified form of full fusion.
Martina JA, Wu XS, Catalfamo M, Sakamoto T, Yi C, Hammer JA 3rd.
Cell Immunol. 2011 01 ;271(2):267-79.
[Text Abstract on PubMed]

Two modes of lytic granule fusion during degranulation by natural killer cells.
Liu D, Martina JA, Wu XS, Hammer JA 3rd, Long EO.
Immunol Cell Biol. 2011 Aug ;89(6):728-38.
[Text Abstract on PubMed]

A new delta-COP on the block--linking pigmentation defects with neurodegeneration.
Wagner W, Hammer JA 3rd.
Pigment Cell Melanoma Res. 2011 Feb ;24(1):14-6.
[Text Abstract on PubMed]

Molecular basis for barbed end uncapping by CARMIL homology domain 3 of mouse CARMIL-1.
Zwolak A, Uruno T, Piszczek G, Hammer, Tjandra N.
J. Biol. Chem. 2010 Sep 10;285(37):29014-26.
[Text Abstract on PubMed]

Structural basis for capping protein sequestration by myotrophin (V-1).
Zwolak A, Fujiwara I, Hammer, Tjandra N.
J. Biol. Chem. 2010 Aug 13;285(33):25767-81.
[Text Abstract on PubMed]

Actin-bundling protein TRIOBP forms resilient rootlets of hair cell stereocilia essential for hearing.
Kitajiri S, Sakamoto T, Belyantseva IA, Goodyear RJ, Stepanyan R, Fujiwara I, Bird JE, Riazuddin S, Riazuddin S, Ahmed ZM, Hinshaw JE, Sellers J, Bartles JR, Hammer, Richardson GP, Griffith AJ, Frolenkov GI, Friedman TB.
Cell. 2010 May 28;141(5):786-98.
[Text Abstract on PubMed]

Direct observation of the uncapping of capping protein-capped actin filaments by CARMIL homology domain 3.
Fujiwara I, Remmert K, Hammer.
J. Biol. Chem. 2010 Jan 22;285(4):2707-20.
[Text Abstract on PubMed]

Linking molecular motors to membrane cargo.
Akhmanova A, Hammer JA 3rd.
Curr Opin Cell Biol. 2010 Aug ;22(4):479-87.
[Text Abstract on PubMed]

Dominant-negative myosin Va impairs retrograde but not anterograde axonal transport of large dense core vesicles.
Bittins CM, Eichler TW, Hammer JA 3rd, Gerdes HH.
Cell Mol Neurobiol. 2010 Apr ;30(3):369-79.
[Text Abstract on PubMed]

The Dictyostelium type V myosin MyoJ is responsible for the cortical association and motility of contractile vacuole membranes.
Jung G, Titus MA, Hammer.
J. Cell Biol. 2009 Aug 24;186(4):555-70.
[Text Abstract on PubMed]

Purification of capping protein using the capping protein binding site of CARMIL as an affinity matrix.
Remmert K, Uruno T, Hammer JA 3rd.
Protein Expr Purif. 2009 Oct ;67(2):113-9.
[Text Abstract on PubMed]

Calcium and cargoes as regulators of myosin 5a activity.
Sellers JR, Thirumurugan K, Sakamoto T, Hammer JA 3rd, Knight PJ.
Biochem Biophys Res Commun. 2008 Apr 25;369(1):176-81.
[Text Abstract on PubMed]

Role of Myosin Va in the plasticity of the vertebrate neuromuscular junction in vivo.
Röder IV, Petersen Y, Choi KR, Witzemann V, Hammer JA 3rd, Rudolf R.
PLoS One. 2008 01 ;3(12):e3871.
[Text Abstract on PubMed]

Organelle motility: running on unleadened.
Hammer JA, Wu XS.
Curr. Biol. 2007 Dec 4;17(23):R1017-9.
[Text Abstract on PubMed]

Slip sliding away with myosin V.
Hammer JA 3rd, Wu X.
Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5255-6.
[Text Abstract on PubMed]

A Role of myosin Vb and Rab11-FIP2 in the aquaporin-2 shuttle.
Nedvetsky PI, Stefan E, Frische S, Santamaria K, Wiesner B, Valenti G, Hammer JA 3rd, Nielsen S, Goldenring JR, Rosenthal W, Klussmann E.
Traffic. 2007 Feb ;8(2):110-23.
[Text Abstract on PubMed]

In vitro reconstitution of a transport complex containing Rab27a, melanophilin and myosin Va.
Wu X, Sakamoto T, Zhang F, Sellers JR, Hammer.
FEBS Lett. 2006 Oct 30;580(25):5863-8.
[Text Abstract on PubMed]

The binding of DYNLL2 to myosin Va requires alternatively spliced exon B and stabilizes a portion of the myosin's coiled-coil domain.
Wagner W, Fodor E, Ginsburg A, Hammer JA 3rd.
Biochemistry. 2006 Sep 26;45(38):11564-77.
[Text Abstract on PubMed]

The cargo-binding domain regulates structure and activity of myosin 5.
Thirumurugan K, Sakamoto T, Hammer, Sellers JR, Knight PJ.
Nature. 2006 Jul 13;442(7099):212-5.
[Text Abstract on PubMed]

CARMIL is a potent capping protein antagonist: identification of a conserved CARMIL domain that inhibits the activity of capping protein and uncaps capped actin filaments.
Uruno T, Remmert K, Hammer JA 3rd.
J Biol Chem. 2006 Apr 14;281(15):10635-50.
[Text Abstract on PubMed]

Motor proteins at the microtubule plus-end.
Wu X, Xiang X, Hammer JA 3rd.
Trends Cell Biol. 2006 Mar ;16(3):135-43.
[Text Abstract on PubMed]

Melanophilin and myosin Va track the microtubule plus end on EB1.
Wu XS, Tsan GL, Hammer JA 3rd.
J Cell Biol. 2005 Oct 24;171(2):201-7.
[Text Abstract on PubMed]

dsu functions in a MYO5A-independent pathway to suppress the coat color of dilute mice.
O'Sullivan TN, Wu XS, Rachel RA, Huang JD, Swing DA, Matesic LE, Hammer JA 3rd, Copeland NG, Jenkins NA.
Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16831-6.
[Text Abstract on PubMed]

Nanometer localization of single green fluorescent proteins: evidence that myosin V walks hand-over-hand via telemark configuration.
Snyder GE, Sakamoto T, Hammer, Sellers JR, Selvin PR.
Biophys. J. 2004 Sep;87(3):1776-83.
[Text Abstract on PubMed]

CARMIL is a bona fide capping protein interactant.
Remmert K, Olszewski TE, Bowers MB, Dimitrova M, Ginsburg A, Hammer.
J. Biol. Chem. 2004 Jan 23;279(4):3068-77.
[Text Abstract on PubMed]

Regulated conformation of myosin V.
Wang F, Thirumurugan K, Stafford WF, Hammer, Knight PJ, Sellers JR.
J. Biol. Chem. 2004 Jan 23;279(4):2333-6.
[Text Abstract on PubMed]

Myosin V and the endoplasmic reticulum: the connection grows.
Wagner W, Hammer.
J. Cell Biol. 2003 Dec 22;163(6):1193-6.
[Text Abstract on PubMed]

Myosin Va facilitates the distribution of secretory granules in the F-actin rich cortex of PC12 cells.
Rudolf R, Kögel T, Kuznetsov SA, Salm T, Schlicker O, Hellwig A, Hammer, Gerdes HH.
J. Cell. Sci. 2003 Apr 1;116(Pt 7):1339-48.
[Text Abstract on PubMed]

Rab27a is an essential component of melanosome receptor for myosin Va.
Wu X, Wang F, Rao K, Sellers JR, Hammer.
Mol. Biol. Cell. 2002 May;13(5):1735-49.
[Text Abstract on PubMed]

Mutation of melanosome protein RAB38 in chocolate mice.
Loftus SK, Larson DM, Baxter LL, Antonellis A, Chen Y, Wu X, Jiang Y, Bittner M, Hammer, Pavan WJ.
Proc. Natl. Acad. Sci. U.S.A. 2002 Apr 2;99(7):4471-6.
[Text Abstract on PubMed]

Identification of an organelle receptor for myosin-Va.
Wu XS, Rao K, Zhang H, Wang F, Sellers JR, Matesic LE, Copeland NG, Jenkins NA, Hammer.
Nat. Cell Biol. 2002 Apr;4(4):271-8.
[Text Abstract on PubMed]

Rabs grab motors: defining the connections between Rab GTPases and motor proteins.
Hammer, Wu XS.
Curr. Opin. Cell Biol. 2002 Feb;14(1):69-75.
[Text Abstract on PubMed]

Influence of alpha-melanocyte-stimulating hormone and ultraviolet radiation on the transfer of melanosomes to keratinocytes.
Virador VM, Muller J, Wu X, Abdel-Malek ZA, Yu ZX, Ferrans VJ, Kobayashi N, Wakamatsu K, Ito S, Hammer JA, Hearing VJ.
FASEB J. 2002 Jan;16(1):105-7.
[Text Abstract on PubMed]

Myosin Va bound to phagosomes binds to F-actin and delays microtubule-dependent motility.
Al-Haddad A, Shonn MA, Redlich B, Blocker A, Burkhardt JK, Yu H, Hammer, Weiss DG, Steffen W, Griffiths G, Kuznetsov SA.
Mol. Biol. Cell. 2001 Sep;12(9):2742-55.
[Text Abstract on PubMed]

The Dictyostelium CARMIL protein links capping protein and the Arp2/3 complex to type I myosins through their SH3 domains.
Jung G, Remmert K, Wu X, Volosky JM, Hammer.
J. Cell Biol. 2001 Jun 25;153(7):1479-97.
[Text Abstract on PubMed]

Glutamate receptor targeting in the postsynaptic spine involves mechanisms that are independent of myosin Va.
Petralia RS, Wang YX, Sans N, Worley PF, Hammer, Wenthold RJ.
Eur. J. Neurosci. 2001 May;13(9):1722-32.
[Text Abstract on PubMed]

Rab27a enables myosin Va-dependent melanosome capture by recruiting the myosin to the organelle.
Wu X, Rao K, Bowers MB, Copeland NG, Jenkins NA, Hammer.
J. Cell. Sci. 2001 Mar;114(Pt 6):1091-100.
[Text Abstract on PubMed]

Defective granule exocytosis in Rab27a-deficient lymphocytes from Ashen mice.
Haddad EK, Wu X, Hammer, Henkart PA.
J. Cell Biol. 2001 Feb 19;152(4):835-42.
[Text Abstract on PubMed]

Making sense of melanosome dynamics in mouse melanocytes.
Wu X, Hammer.
Pigment Cell Res. 2000 Aug;13(4):241-7.
[Text Abstract on PubMed]

Two-headed binding of a processive myosin to F-actin.
Walker ML, Burgess SA, Sellers JR, Wang F, Hammer, Trinick J, Knight PJ.
Nature. 2000 Jun 15;405(6788):804-7.
[Text Abstract on PubMed]

Effect of ADP and ionic strength on the kinetic and motile properties of recombinant mouse myosin V.
Wang F, Chen L, Arcucci O, Harvey EV, Bowers B, Xu Y, Hammer, Sellers JR.
J. Biol. Chem. 2000 Feb 11;275(6):4329-35.
[Text Abstract on PubMed]

Functions of unconventional myosins.
Wu X, Jung G, Hammer.
Curr. Opin. Cell Biol. 2000 Feb;12(1):42-51.
[Text Abstract on PubMed]

Flexibility of Acanthamoeba myosin rod minifilaments.
Redowicz MJ, Hammer, Bowers B, Zolkiewski M, Ginsburg A, Korn ED, Rau DC.
Biochemistry. 1999 Jun 1;38(22):7243-52.
[Text Abstract on PubMed]

Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function In vivo.
Wu X, Bowers B, Rao K, Wei Q, Hammer JA.
J. Cell Biol. 1998 Dec 28;143(7):1899-918.
[Text Abstract on PubMed]

Analysis of the regulatory phosphorylation site in Acanthamoeba myosin IC by using site-directed mutagenesis.
Wang ZY, Wang F, Sellers JR, Korn ED, Hammer.
Proc. Natl. Acad. Sci. U.S.A. 1998 Dec 22;95(26):15200-5.
[Text Abstract on PubMed]

Structural invariance of constitutively active and inactive mutants of acanthamoeba myosin IC bound to F-actin in the rigor and ADP-bound states.
Carragher BO, Cheng N, Wang ZY, Korn ED, Reilein A, Belnap DM, Hammer, Steven AC.
Proc. Natl. Acad. Sci. U.S.A. 1998 Dec 22;95(26):15206-11.
[Text Abstract on PubMed]

Myosin Va associates with microtubule-rich domains in both interphase and dividing cells.
Wu X, Kocher B, Wei Q, Hammer.
Cell Motil. Cytoskeleton. 1998;40(3):286-303.
[Text Abstract on PubMed]

The predominant defect in dilute melanocytes is in melanosome distribution and not cell shape, supporting a role for myosin V in melanosome transport.
Wei Q, Wu X, Hammer.
J. Muscle Res. Cell. Motil. 1997 Oct;18(5):517-27.
[Text Abstract on PubMed]

Two-state thermal unfolding of a long dimeric coiled-coil: the Acanthamoeba myosin II rod.
Zolkiewski M, Redowicz MJ, Korn ED, Hammer, Ginsburg A.
Biochemistry. 1997 Jun 24;36(25):7876-83.
[Text Abstract on PubMed]

The amino acid sequence of the light chain of Acanthamoeba myosin IC.
Wang ZY, Sakai J, Matsudaira PT, Baines IC, Sellers JR, Hammer, Korn ED.
J. Muscle Res. Cell. Motil. 1997 Jun;18(3):395-8.
[Text Abstract on PubMed]

Myosin V associates with melanosomes in mouse melanocytes: evidence that myosin V is an organelle motor.
Wu X, Bowers B, Wei Q, Kocher B, Hammer.
J. Cell. Sci. 1997 Apr;110 ( Pt 7):847-59.
[Text Abstract on PubMed]

Localization of Dictyostelium myoB and myoD to filopodia and cell-cell contact sites using isoform-specific antibodies.
Morita YS, Jung G, Hammer, Fukui Y.
Eur. J. Cell Biol. 1996 Dec;71(4):371-9.
[Text Abstract on PubMed]

Structural change of the endoplasmic reticulum during fertilization: evidence for loss of membrane continuity using the green fluorescent protein.
Terasaki M, Jaffe LA, Hunnicutt GR, Hammer.
Dev. Biol. 1996 Nov 1;179(2):320-8.
[Text Abstract on PubMed]

Dictyostelium mutants lacking multiple classic myosin I isoforms reveal combinations of shared and distinct functions.
Jung G, Wu X, Hammer.
J. Cell Biol. 1996 Apr;133(2):305-23.
[Text Abstract on PubMed]

The sequence of the dictyostelium myo J heavy chain gene predicts a novel, dimeric, unconventional myosin with a heavy chain molecular mass of 258 kDa.
Hammer, Jung G.
J. Biol. Chem. 1996 Mar 22;271(12):7120-7.
[Text Abstract on PubMed]

Regulation of Dictyostelium myosin II by phosphorylation: what is essential and what is important?
J. Cell Biol. 1994 Dec;127(6 Pt 2):1779-82.
[Text Abstract on PubMed]

The actin binding site in the tail domain of Dictyostelium myosin IC (myoC) resides within the glycine- and proline-rich sequence (tail homology region 2).
Jung G, Hammer.
FEBS Lett. 1994 Apr 4;342(2):197-202.
[Text Abstract on PubMed]

The structure and function of unconventional myosins: a review.
J. Muscle Res. Cell. Motil. 1994 Feb;15(1):1-10.
[Text Abstract on PubMed]

Sequence, expression pattern, intracellular localization, and targeted disruption of the Dictyostelium myosin ID heavy chain isoform.
Jung G, Fukui Y, Martin B, Hammer.
J. Biol. Chem. 1993 Jul 15;268(20):14981-90.
[Text Abstract on PubMed]

The Dictyostelium myosin IE heavy chain gene encodes a truncated isoform that lacks sequences corresponding to the actin binding site in the tail.
Urrutia RA, Jung G, Hammer.
Biochim. Biophys. Acta. 1993 May 28;1173(2):225-9.
[Text Abstract on PubMed]

Novel myosins.
Hammer JA.
Trends Cell Biol. 1991 Aug;1(2-3):50-6.
[Text Abstract on PubMed]

Myosin IB null mutants of Dictyostelium exhibit abnormalities in motility.
Wessels D, Murray J, Jung G, Hammer, Soll DR.
Cell Motil. Cytoskeleton. 1991;20(4):301-15.
[Text Abstract on PubMed]

Molecular cloning of protozoan myosin I heavy chain genes.
Hammer, Jung G.
J. Cell Sci. Suppl. 1991;14:37-40.
[Text Abstract on PubMed]

A new Acanthamoeba myosin heavy chain. Cloning of the gene and immunological identification of the polypeptide.
Horowitz JA, Hammer.
J. Biol. Chem. 1990 Nov 25;265(33):20646-52.
[Text Abstract on PubMed]

A second isoform of chicken brush border myosin I contains a 29-residue inserted sequence that binds calmodulin.
Halsall DJ, Hammer.
FEBS Lett. 1990 Jul 2;267(1):126-30.
[Text Abstract on PubMed]

Generation and characterization of Dictyostelium cells deficient in a myosin I heavy chain isoform.
Jung G, Hammer.
J. Cell Biol. 1990 Jun;110(6):1955-64.
[Text Abstract on PubMed]

Myosin I.
Korn ED, Hammer.
Curr. Opin. Cell Biol. 1990 Feb;2(1):57-61.
[Text Abstract on PubMed]

Myosin I heavy-chain genes of Acanthamoeba castellanii: cloning of a second gene and evidence for the existence of a third isoform.
Jung G, Schmidt CJ, Hammer.
Gene. 1989 Oct 30;82(2):269-80.
[Text Abstract on PubMed]

Dictyostelium discoideum contains a gene encoding a myosin I heavy chain.
Jung G, Saxe, Kimmel AR, Hammer.
Proc. Natl. Acad. Sci. U.S.A. 1989 Aug;86(16):6186-90.
[Text Abstract on PubMed]

Structure-function studies on Acanthamoeba myosins IA, IB, and II.
Korn ED, Atkinson MA, Brzeska H, Hammer, Jung G, Lynch TJ.
J. Cell. Biochem. 1988 Jan;36(1):37-50.
[Text Abstract on PubMed]

Myosins of nonmuscle cells.
Korn ED, Hammer.
Annu Rev Biophys Biophys Chem. 1988;17:23-45.
[Text Abstract on PubMed]

The heavy chain of Acanthamoeba myosin IB is a fusion of myosin-like and non-myosin-like sequences.
Jung G, Korn ED, Hammer.
Proc. Natl. Acad. Sci. U.S.A. 1987 Oct;84(19):6720-4.
[Text Abstract on PubMed]

Genetic evidence that Acanthamoeba myosin I is a true myosin.
Hammer, Jung G, Korn ED.
Proc. Natl. Acad. Sci. U.S.A. 1986 Jul;83(13):4655-9.
[Text Abstract on PubMed]

Isolation of a non-muscle myosin heavy chain gene from Acanthamoeba.
Hammer, Korn ED, Paterson BM.
J. Biol. Chem. 1986 Feb 5;261(4):1949-56.
[Text Abstract on PubMed]

Monomeric Acanthamoeba myosins I support movement in vitro.
Albanesi JP, Fujisaki H, Hammer, Korn ED, Jones R, Sheetz MP.
J. Biol. Chem. 1985 Jul 25;260(15):8649-52.
[Text Abstract on PubMed]

Purification from Dictyostelium discoideum of a low-molecular-weight myosin that resembles myosin I from Acanthamoeba castellanii.
Côté GP, Albanesi JP, Ueno T, Hammer, Korn ED.
J. Biol. Chem. 1985 Apr 25;260(8):4543-6.
[Text Abstract on PubMed]

Acanthamoeba myosin IA, IB, and II heavy chains are synthesized in vitro from Acanthamoeba messenger RNA.
Hammer, Korn ED, Paterson BM.
J. Biol. Chem. 1984 Sep 25;259(18):11157-9.
[Text Abstract on PubMed]

Phosphorylation and activation of smooth muscle myosin by Acanthamoeba myosin I heavy chain kinase.
Hammer, Sellers JR, Korn ED.
J. Biol. Chem. 1984 Mar 10;259(5):3224-9.
[Text Abstract on PubMed]

The interaction of F-actin with phosphorylated and unphosphorylated myosins IA and IB from Acanthamoeba castellanii.
Albanesi JP, Hammer, Korn ED.
J. Biol. Chem. 1983 Aug 25;258(16):10176-81.
[Text Abstract on PubMed]

Purification and characterization of a myosin I heavy chain kinase from Acanthamoeba castellanii.
Hammer, Albanesi JP, Korn ED.
J. Biol. Chem. 1983 Aug 25;258(16):10168-75.
[Text Abstract on PubMed]

Protein turnover in pulmonary macrophages. Utilization of amino acids derived from protein degradation.
Hammer, Rannels DE.
Biochem. J. 1981 Jul 15;198(1):53-65.
[Text Abstract on PubMed]

Effects of halothane on protein synthesis and degradation in rabbit pulmonary macrophages.
Hammer, Rannels DE.
Am. Rev. Respir. Dis. 1981 Jul;124(1):50-5.
[Text Abstract on PubMed]