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Proteomics Core


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Proteomics Core
Marjan Gucek, Ph.D.

Full Publications Listing

Global analysis of neuronal phosphoproteome regulation by chondroitin sulfate proteoglycans.
Yu P, Pisitkun T, Wang G, Wang R, Katagiri Y, Gucek M, Knepper MA, Geller HM.
PLoS One. 2013 01;8(3):e59285.
[Text Abstract on PubMed]

Brefeldin A-inhibited ADP-ribosylation factor activator BIG2 regulates cell migration via integrin ß1 cycling and actin remodeling.
Shen X, Li CC, Aponte AM, Shen RF, Billings EM, Moss J, Vaughan M.
Proc. Natl. Acad. Sci. U.S.A. 2012 Sep 4;109(36):14464-9.
[Text Abstract on PubMed]

Essential role of nitric oxide in acute ischemic preconditioning: S-Nitros(yl)ation versus sGC/cGMP/PKG signaling?
Sun J, Aponte AM, Kohr MJ, Tong G, Steenbergen C, Murphy E.
Free Radic. Biol. Med. 2012 Sep 16.
[Text Abstract on PubMed]

Measurement of s-nitrosylation occupancy in the myocardium with cysteine-reactive tandem mass tags: short communication.
Kohr MJ, Aponte A, Sun J, Gucek M, Steenbergen C, Murphy E.
Circ. Res. 2012 Oct 26;111(10):1308-12.
[Text Abstract on PubMed]

Phosphorylation of claudin-2 on serine 208 promotes membrane retention and reduces trafficking to lysosomes.
Van Itallie CM, Tietgens AJ, Logrande K, Aponte A, Gucek M, Anderson JM.
J. Cell. Sci. 2012 Oct 15;125(Pt 20):4902-4912.
[Text Abstract on PubMed]

Quantitative proteomics of parotid saliva in primary Sjögren's syndrome.
Ambatipudi KS, Swatkoski S, Moresco JJ, Tu PG, Coca A, Anolik JH, Gucek M, Sanz I, Yates, Melvin JE.
Proteomics. 2012 Oct;12(19-20):3113-20.
[Text Abstract on PubMed]

Proteomic analysis of high NaCl-induced changes in abundance of nuclear proteins.
Li J, Ferraris JD, Yu D, Singh T, Izumi Y, Wang G, Gucek M, Burg MB.
Physiol. Genomics. 2012 Nov 2;44(21):1063-71.
[Text Abstract on PubMed]

Regulation of oxidative phosphorylation complex activity: effects of tissue-specific metabolic stress within an allometric series and acute changes in workload.
Phillips D, Covian R, Aponte AM, Glancy B, Taylor JF, Chess D, Balaban RS.
Am. J. Physiol. Regul. Integr. Comp. Physiol. 2012 May;302(9):R1034-48.
[Text Abstract on PubMed]

Acute inhibition of GSK causes mitochondrial remodeling.
Nguyen T, Wong R, Wang G, Gucek M, Steenbergen C, Murphy E.
Am. J. Physiol. Heart Circ. Physiol. 2012 Jun 1;302(11):H2439-45.
[Text Abstract on PubMed]

Quantitative proteomics identifies vasopressin-responsive nuclear proteins in collecting duct cells.
Schenk LK, Bolger SJ, Luginbuhl K, Gonzales PA, Rinschen MM, Yu MJ, Hoffert JD, Pisitkun T, Knepper MA.
J. Am. Soc. Nephrol. 2012 Jun;23(6):1008-18.
[Text Abstract on PubMed]

MTORC1 functions as a transcriptional regulator of autophagy by preventing nuclear transport of TFEB.
Martina JA, Chen Y, Gucek M, Puertollano R.
Autophagy. 2012 Jun;8(6):903-14.
[Text Abstract on PubMed]

Quantitative Salivary Proteomic Differences in Oral Chronic Graft-versus-Host Disease.
Bassim CW, Ambatipudi KS, Mays JW, Edwards DA, Swatkoski S, Fassil H, Baird K, Gucek M, Melvin JE, Pavletic SZ.
J. Clin. Immunol. 2012 Jul 18.
[Text Abstract on PubMed]

Disruption of caveolae blocks ischemic preconditioning-mediated S-nitrosylation of mitochondrial proteins.
Sun J, Kohr MJ, Nguyen T, Aponte AM, Connelly PS, Esfahani SG, Gucek M, Daniels MP, Steenbergen C, Murphy E.
Antioxid. Redox Signal. 2012 Jan 1;16(1):45-56.
[Text Abstract on PubMed]

Large-scale phosphotyrosine proteomic profiling of rat renal collecting duct epithelium reveals predominance of proteins involved in cell polarity determination.
Zhao B, Knepper MA, Chou CL, Pisitkun T.
Am. J. Physiol., Cell Physiol. 2012 Jan 1;302(1):C27-45.
[Text Abstract on PubMed]

High-density lipoprotein cholesterol efflux, nitration of apolipoprotein A-I, and endothelial function in obese women.
Vazquez E, Sethi AA, Freeman L, Zalos G, Chaudhry H, Haser E, Aicher BO, Aponte A, Gucek M, Kato GJ, Waclawiw MA, Remaley AT, Cannon.
Am. J. Cardiol. 2012 Feb 15;109(4):527-32.
[Text Abstract on PubMed]

Dynamics of the G protein-coupled vasopressin V2 receptor signaling network revealed by quantitative phosphoproteomics.
Hoffert JD, Pisitkun T, Saeed F, Song JH, Chou CL, Knepper MA.
Mol. Cell Proteomics. 2012 Feb;11(2):M111.014613.
[Text Abstract on PubMed]

Two protein lysine methyltransferases methylate outer membrane protein B from rickettsia.
Abeykoon AH, Chao CC, Wang G, Gucek M, Yang DC, Ching WM.
J. Bacteriol. 2012 Dec;194(23):6410-8.
[Text Abstract on PubMed]

Role of mitochondrial Ca2+ in the regulation of cellular energetics.
Glancy B, Balaban RS.
Biochemistry. 2012 Apr 10;51(14):2959-73.
[Text Abstract on PubMed]

Platelet mitochondrial dysfunction is evident in type 2 diabetes in association with modifications of mitochondrial anti-oxidant stress proteins.
Avila C, Huang RJ, Stevens MV, Aponte AM, Tripodi D, Kim KY, Sack MN.
Exp. Clin. Endocrinol. Diabetes. 2012 Apr;120(4):248-51.
[Text Abstract on PubMed]

Homogenous protein programming in the mammalian left and right ventricle free walls.
Phillips D, Aponte AM, Covian R, Neufeld E, Yu ZX, Balaban RS.
Physiol. Genomics. 2011 Nov 7;43(21):1198-206.
[Text Abstract on PubMed]

Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore.
Nguyen TT, Stevens MV, Kohr M, Steenbergen C, Sack MN, Murphy E.
J. Biol. Chem. 2011 Nov 18;286(46):40184-92.
[Text Abstract on PubMed]

Sorting nexin 27 protein regulates trafficking of a p21-activated kinase (PAK) interacting exchange factor (ß-Pix)-G protein-coupled receptor kinase interacting protein (GIT) complex via a PDZ domain interaction.
Valdes JL, Tang J, McDermott MI, Kuo JC, Zimmerman SP, Wincovitch SM, Waterman CM, Milgram SL, Playford MP.
J. Biol. Chem. 2011 Nov 11;286(45):39403-16.
[Text Abstract on PubMed]

High NaCl-induced activation of CDK5 increases phosphorylation of the osmoprotective transcription factor TonEBP/OREBP at threonine 135, which contributes to its rapid nuclear localization.
Gallazzini M, Heussler GE, Kunin M, Izumi Y, Burg MB, Ferraris JD.
Mol. Biol. Cell. 2011 Mar 1;22(5):703-14.
[Text Abstract on PubMed]

Hydrolysis of O-acetyl-ADP-ribose isomers by ADP-ribosylhydrolase 3.
Kasamatsu A, Nakao M, Smith BC, Comstock LR, Ono T, Kato J, Denu JM, Moss J.
J. Biol. Chem. 2011 Jun 17;286(24):21110-7.
[Text Abstract on PubMed]

Protein composition and function of red and white skeletal muscle mitochondria.
Glancy B, Balaban RS.
Am. J. Physiol., Cell Physiol. 2011 Jun;300(6):C1280-90.
[Text Abstract on PubMed]

Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.
Khositseth S, Pisitkun T, Slentz DH, Wang G, Hoffert JD, Knepper MA, Yu MJ.
Mol. Cell Proteomics. 2011 Jan;10(1):M110.004036.
[Text Abstract on PubMed]

Simultaneous measurement of protein oxidation and S-nitrosylation during preconditioning and ischemia/reperfusion injury with resin-assisted capture.
Kohr MJ, Sun J, Aponte A, Wang G, Gucek M, Murphy E, Steenbergen C.
Circ. Res. 2011 Feb 18;108(4):418-26.
[Text Abstract on PubMed]

SIRT3-dependent deacetylation exacerbates acetaminophen hepatotoxicity.
Lu Z, Bourdi M, Li JH, Aponte AM, Chen Y, Lombard DB, Gucek M, Pohl LR, Sack MN.
EMBO Rep. 2011 Aug;12(8):840-6.
[Text Abstract on PubMed]

Intrinsic protein kinase activity in mitochondrial oxidative phosphorylation complexes.
Phillips D, Aponte AM, Covian R, Balaban RS.
Biochemistry. 2011 Apr 5;50(13):2515-29.
[Text Abstract on PubMed]

Fox-3 and PSF interact to activate neural cell-specific alternative splicing.
Kim KK, Kim YC, Adelstein RS, Kawamoto S.
Nucleic Acids Res. 2011 Apr;39(8):3064-78.
[Text Abstract on PubMed]

Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule.
Feric M, Zhao B, Hoffert JD, Pisitkun T, Knepper MA.
Am. J. Physiol., Cell Physiol. 2011 Apr;300(4):C755-70.
[Text Abstract on PubMed]

Characterization of potential S-nitrosylation sites in the myocardium.
Kohr MJ, Aponte AM, Sun J, Wang G, Murphy E, Gucek M, Steenbergen C.
Am. J. Physiol. Heart Circ. Physiol. 2011 Apr;300(4):H1327-35.
[Text Abstract on PubMed]

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ß-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates, Waterman CM.
Nat. Cell Biol. 2011 Apr;13(4):383-93.
[Text Abstract on PubMed]

MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins.
Vickers KC, Palmisano BT, Shoucri BM, Shamburek RD, Remaley AT.
Nat. Cell Biol. 2011 Apr;13(4):423-33.
[Text Abstract on PubMed]

Ablation of nonmuscle myosin II-B and II-C reveals a role for nonmuscle myosin II in cardiac myocyte karyokinesis.
Ma X, Jana SS, Conti MA, Kawamoto S, Claycomb WC, Adelstein RS.
Mol. Biol. Cell. 2010 Nov 15;21(22):3952-62.
[Text Abstract on PubMed]

What can we learn about cardioprotection from the cardiac mitochondrial proteome?
Gucek M, Murphy E.
Cardiovasc. Res. 2010 Nov 1;88(2):211-8.
[Text Abstract on PubMed]

Sex differences in the phosphorylation of mitochondrial proteins result in reduced production of reactive oxygen species and cardioprotection in females.
Lagranha CJ, Deschamps A, Aponte A, Steenbergen C, Murphy E.
Circ. Res. 2010 Jun 11;106(11):1681-91.
[Text Abstract on PubMed]

Proteomic analysis of V-ATPase-rich cells harvested from the kidney and epididymis by fluorescence-activated cell sorting.
Da Silva N, Pisitkun T, Belleannée C, Miller LR, Nelson R, Knepper MA, Brown D, Breton S.
Am. J. Physiol., Cell Physiol. 2010 Jun;298(6):C1326-42.
[Text Abstract on PubMed]

Stoichiometry of STAT3 and mitochondrial proteins: Implications for the regulation of oxidative phosphorylation by protein-protein interactions.
Phillips D, Reilley MJ, Aponte AM, Wang G, Boja E, Gucek M, Balaban RS.
J. Biol. Chem. 2010 Jul 30;285(31):23532-6.
[Text Abstract on PubMed]

Cardioprotection leads to novel changes in the mitochondrial proteome.
Wong R, Aponte AM, Steenbergen C, Murphy E.
Am. J. Physiol. Heart Circ. Physiol. 2010 Jan;298(1):H75-91.
[Text Abstract on PubMed]

Proteomic profiling of nuclei from native renal inner medullary collecting duct cells using LC-MS/MS.
Tchapyjnikov D, Li Y, Pisitkun T, Hoffert JD, Yu MJ, Knepper MA.
Physiol. Genomics. 2010 Feb 4;40(3):167-83.
[Text Abstract on PubMed]

Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells.
Rinschen MM, Yu MJ, Wang G, Boja ES, Hoffert JD, Pisitkun T, Knepper MA.
Proc. Natl. Acad. Sci. U.S.A. 2010 Feb 23;107(8):3882-7.
[Text Abstract on PubMed]

Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting duct.
Bansal AD, Hoffert JD, Pisitkun T, Hwang S, Chou CL, Boja ES, Wang G, Knepper MA.
J. Am. Soc. Nephrol. 2010 Feb;21(2):303-15.
[Text Abstract on PubMed]

Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cells.
Gunaratne R, Braucht DW, Rinschen MM, Chou CL, Hoffert JD, Pisitkun T, Knepper MA.
Proc. Natl. Acad. Sci. U.S.A. 2010 Aug 31;107(35):15653-8.
[Text Abstract on PubMed]

Mice over-expressing the myocardial creatine transporter develop progressive heart failure and show decreased glycolytic capacity.
Phillips D, Ten Hove M, Schneider JE, Wu CO, Sebag-Montefiore L, Aponte AM, Lygate CA, Wallis J, Clarke K, Watkins H, Balaban RS, Neubauer S.
J. Mol. Cell. Cardiol. 2010 Apr;48(4):582-90.
[Text Abstract on PubMed]

Mediator of DNA damage checkpoint 1 (MDC1) contributes to high NaCl-induced activation of the osmoprotective transcription factor TonEBP/OREBP.
Kunin M, Dmitrieva NI, Gallazzini M, Shen RF, Wang G, Burg MB, Ferraris JD.
PLoS ONE. 2010;5(8):e12108.
[Text Abstract on PubMed]

Proteomic changes associated with diabetes in the BB-DP rat.
Johnson DT, Harris RA, French S, Aponte A, Balaban RS.
Am. J. Physiol. Endocrinol. Metab. 2009 Mar;296(3):E422-32.
[Text Abstract on PubMed]

Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry.
Zwang NA, Hoffert JD, Pisitkun T, Moeller HB, Fenton RA, Knepper MA.
J. Proteome Res. 2009 Mar;8(3):1540-54.
[Text Abstract on PubMed]

Use of (32)P to study dynamics of the mitochondrial phosphoproteome.
Aponte AM, Phillips D, Hopper RK, Johnson DT, Harris RA, Blinova K, Boja ES, French S, Balaban RS.
J. Proteome Res. 2009 Jun;8(6):2679-95.
[Text Abstract on PubMed]

Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct.
Yu MJ, Miller RL, Uawithya P, Rinschen MM, Khositseth S, Braucht DW, Chou CL, Pisitkun T, Nelson RD, Knepper MA.
Proc. Natl. Acad. Sci. U.S.A. 2009 Feb 17;106(7):2441-6.
[Text Abstract on PubMed]

Succinyl-CoA synthetase is a phosphate target for the activation of mitochondrial metabolism.
Phillips D, Aponte AM, French SA, Chess DJ, Balaban RS.
Biochemistry. 2009 Aug 4;48(30):7140-9.
[Text Abstract on PubMed]

32P labeling of protein phosphorylation and metabolite association in the mitochondria matrix.
Aponte AM, Phillips D, Harris RA, Blinova K, French S, Johnson DT, Balaban RS.
Meth. Enzymol. 2009;457:63-80.
[Text Abstract on PubMed]

Cyclophilin D Modulates the Mitochondrial Acetylome.
Nguyen TT, Wong RP, Menazza S, Sun J, Chen Y, Wang G, Gucek M, Steenbergen C, Sack MN, Murphy E.
Circ Res..
[Text Abstract on PubMed]