Light Microscopy Core

The Light Microscopy Core provides state-of-the-art equipment, training, and image processing capabilities to assist researchers in experiments involving light microscopy. Equipment within the facility includes several types of confocal microscopes, two multi-photon microscope, an FCS microscope, laser trap microscope, TIRF microscopes, several standard epi-fluorescence wide-field microscopes and specialty microscopes for super-resolution imaging and wide-area confocal imaging. This range of instruments allows for most types of modern imaging techniques from basic wide-field imaging to very high spatial and temporal resolution imaging for fixed and live-cell imaging to imaging organs and tissues in vivo. Image processing capabilities include image quantification, co-localization analysis, deconvolution, and 3D reconstruction as well as a custom in-house image processing programs for specific applications. The main points of contact in Bldg. 10 are Drs. Combs and Malide and Dr. Wu in Bldg. 50.

Instruments

Bldg. 10

• Leica SP5 Multi-Photon (dual beam with OPO)/Confocal Microscope
• Zeiss LSM 510 META Confocal Microscope
• Zeiss LSM 510 UV Confocal Microscope
• Nikon Widefield Fluorescence Microscope
• Leica Fluorescence Stereomicroscope
• Modified Olympus IX81 Microscope (Structured Light, Ratiometric Calcium, Dual-beam TIRF Microscopy)
• Leica SP1 Confocal Microscope
• Leica TCS LSI Super-Zoom Confocal
• Olympus Fluoview 1000 Experimental Microcope

Bldg. 50 

• Zeiss LSM 510 confocorr2 (FCS) Microscope
• Zeiss inverted Axiovert 100 Microscope with microinjector
• Zeiss LSM 510 META Confocal Microscope
• Zeiss  5 LIVE Confocal  Microscope
• Zeiss LSM 780 Microscope
• Zeiss Stereomicroscope  (SteREO Discovery V12)
• Zeiss up-right Axioplan Microscope
• Olympus IX 81 TIRF microscope
• Olympus XI81 with CSU-X1 spinning disk confocal and customer-made Laser Trap
• Zeiss LSM  710 2P
• Nikon STORM Super-resolutionMicroscope
• Coming soon: DeltaVison OMX Structured Light Microscope

Christian Combs
Core Director
Light Microscopy Core

P: +1 301 496 3236
F: +1 301 480 1477
combsc@nhlbi.nih.gov

Xufeng Wu
Deputy Core Director
Light Microscopy Core

P: +1 301 402 4187
F: +1 301 402 1519
wux@nhlbi.nih.gov

Daniela Malide
Staff Scientist
Light Microscopy Core

P: +1 301 402 4719
F: +1 301 480 1477
dm343o@nih.gov