The Biophysics Core Facility provides support in the study of macromolecular interactions, dynamics, and stability. We develop and optimize measurement methods as well as provide consultations, training, professional collaborations, and instrument access for NHLBI and other NIH institutes.
The Biophysics Core Facility has the resources and expertise to study oligomeric states of biomolecular assemblies and to perform measurements of affinity, stoichiometry, kinetics, and thermodynamics of interactions between proteins, DNA, RNA, and their cognate ligands. The Core Facility is also equipped with instrumentation for characterization of nanoparticles and macromolecules. Biophysical characterization capabilities include measurements of molecular weight, shape, and conformation of biological macromolecules.
- Isothermal Titration Calorimetry (ITC: iTC200 and VP-ITC)
- Differential Scanning Calorimetry (DSC: VP-DSC and Nano-DSC)
- Surface Plasmon Resonance (SPR: Biacore 3000)
- Bio-Layer Interferometry (BLI: Octet RED96)
- MicroScale Thermophoresis (MST: Monolith NT.115)
- Analytical Ultracentrifugation (AUC: XLA and XLI)
- Multi Angle Light Scattering (SEC-MALS/QELS: DAWN Helos II)
- Dynamic Light Scattering (DLS: Dynapro Nanostar)
- Circular Dichroism (CD: Jasco J715)
- Fluorescence (Photon Technology)