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Biochemistry Core

The Biochemistry Core provides expert services and consultation in biochemical enzyme assays and protein purification.  The Core currently features 1) Liquid chromatography – mass spectrometry (LC-MS) to detect the accurate mass of intact proteins, 2) High performance liquid chromatography (HPLC)  equipped with radiochemical detectors and fluorescence detectors that allow for the purification of an enzyme or protein labeled with a radioisotope or fluorescence probe, and 3) Atomic absorption spectrometry to measure metal content.

After training by the Core staff, users can access equipment on a walk-in basis or may reserve time in advance.  The reservation scheduler can be accessed here [link] or by emailing the Core staff with the requested date and time.

Instruments

  • ESI-LC/MS, Agilent 6224 TOF and G1946D SQ
  • Radiochemical detector, PerkinElmer Radiomatic 625TR w/ Agilent 1100 HPLC
  • Fluorescence detector  w/ Agilent 1100 HPLC
  • Agilent 3100 OFFGel Fractionator
  • Atomic absorption spectrometer, PerkinElmer graphite furnace 4100ZL
  • Bio-Rad open column system
  • Liquid scintillation analyzer, PerkinElmer Tri-Carb 2900TR
  • Anaerobic chamber


Core Staff

The Core staff members are experts in biochemical enzyme assays and protein purification.  They are happy to provide advice and training as necessary.

 

Duck-Yeon Lee
Core Director
Biochemistry Core

P: +1 301 435 8369
F: +1 301 451 5459
leedy@nhlbi.nih.gov


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